The Ohio State University Ohio State Biochemistry Program (OSBP)

Stella Lai

Areas of Expertise

Chemistry & Biochemistry

Education

MS, The Ohio State University, 2017
BA, University of Chicago, 2009

Publications

Kibler RD, Lee S, Kennedy MA, Wicky BIM, Lai SM, Kostelic MM, Carr A, Li X, Chow CM, Nguyen TK, Carter L, Wysocki VH, Stoddard BL, Baker D. Design of pseudosymmetric protein hetero-oligomers. Nat Commun. 2024 Dec 18;15(1):10684. doi: 10.1038/s41467-024-54913-8. PMID: 39695145; PMCID: PMC11655659.
Witzel MT, Veltri LM, Kostelic M, Elshamy YS, Lucas JA, Lai S, Du C, Wysocki VH, Holland LA. Protein analysis using capillary electrophoresis coupled to mass spectrometry through vibrating sharp-edge spray ionization. Electrophoresis. 2024 Sep;45(17-18):1597-1605. doi: 10.1002/elps.202300298. Epub 2024 Apr 5. PMID: 38577828; PMCID: PMC11438567.
Lai LB, Lai SM, Szymanski ES, Kapur M, Choi EK, Al-Hashimi HM, Ackerman SL, Gopalan V. Structural basis for impaired 5' processing of a mutant tRNA associated with defects in neuronal homeostasis. Proc Natl Acad Sci U S A. 2022 Mar 8;119(10):e2119529119. doi: 10.1073/pnas.2119529119. Epub 2022 Mar 1. PMID: 35238631; PMCID: PMC8915964.
Zahurancik WJ, Norris AS, Lai SM, Snyder DT, Wysocki VH, Gopalan V. Purification, reconstitution, and mass analysis of archaeal RNase P, a multisubunit ribonucleoprotein enzyme. Methods Enzymol. 2021;659:71-103. doi: 10.1016/bs.mie.2021.07.006. Epub 2021 Sep 25. PMID: 34752299; PMCID: PMC9099236.
Lai SM, Thirugnanasambantham P, Sidharthan V, Norris AS, Law JD, Gopalan V, Wysocki VH. Use of tandem affinity-buffer exchange chromatography online with native mass spectrometry for optimizing overexpression and purification of recombinant proteins. Methods Enzymol. 2021;659:37-70. doi: 10.1016/bs.mie.2021.07.007. Epub 2021 Sep 23. PMID: 34752295; PMCID: PMC9055603.
Baniasad M, Reed AJ, Lai SM, Zhang L, Schulte KQ, Smith AR, LeSassier DS, Weber KL, Hewitt FC, Woerner AE, Gardner MW, Wysocki VH, Freitas MA. Optimization of proteomics sample preparation for forensic analysis of skin samples. J Proteomics. 2021 Oct 30;249:104360. doi: 10.1016/j.jprot.2021.104360. Epub 2021 Sep 1. PMID: 34481086.
Marathe IA, Lai SM, Zahurancik WJ, Poirier MG, Wysocki VH, Gopalan V. Protein cofactors and substrate influence Mg2+-dependent structural changes in the catalytic RNA of archaeal RNase P. Nucleic Acids Res. 2021 Sep 20;49(16):9444-9458. doi: 10.1093/nar/gkab655. PMID: 34387688; PMCID: PMC8450104.
Busch F, VanAernum ZL, Lai SM, Gopalan V, Wysocki VH. Analysis of Tagged Proteins Using Tandem Affinity-Buffer Exchange Chromatography Online with Native Mass Spectrometry. Biochemistry. 2021 Jun 22;60(24):1876-1884. doi: 10.1021/acs.biochem.1c00138. Epub 2021 Jun 8. PMID: 34100589; PMCID: PMC9080447.
Lai SM, Gopalan V. Using an L7Ae-Tethered, Hydroxyl Radical-Mediated Footprinting Strategy to Identify and Validate Kink-Turns in RNAs. Methods Mol Biol. 2021;2167:147-169. doi: 10.1007/978-1-0716-0716-9_9. Erratum in: Methods Mol Biol. 2021;2167:C1. doi: 10.1007/978-1-0716-0716-9_17. PMID: 32712919.
Agu CV, Lai SM, Ujor V, Biswas PK, Jones A, Gopalan V, Ezeji TC. Development of a high-throughput assay for rapid screening of butanologenic strains. Sci Rep. 2018 Feb 21;8(1):3379. doi: 10.1038/s41598-017-18074-7. PMID: 29467394; PMCID: PMC5821843.
Lai LB, Tanimoto A, Lai SM, Chen WY, Marathe IA, Westhof E, Wysocki VH, Gopalan V. A novel double kink-turn module in euryarchaeal RNase P RNAs. Nucleic Acids Res. 2017 Jul 7;45(12):7432-7440. doi: 10.1093/nar/gkx388. PMID: 28525600; PMCID: PMC5499556.
Samanta MP, Lai SM, Daniels CJ, Gopalan V. Sequence Analysis and Comparative Study of the Protein Subunits of Archaeal RNase P. Biomolecules. 2016 Apr 20;6(2):22. doi: 10.3390/biom6020022. PMID: 27104580; PMCID: PMC4919917.
Lai SM, Lai LB, Foster MP, Gopalan V. The L7Ae protein binds to two kink-turns in the Pyrococcus furiosus RNase P RNA. Nucleic Acids Res. 2014 Dec 1;42(21):13328-38. doi: 10.1093/nar/gku994. Epub 2014 Oct 31. PMID: 25361963; PMCID: PMC4245976.
Ma X, Lai LB, Lai SM, Tanimoto A, Foster MP, Wysocki VH, Gopalan V. Uncovering the stoichiometry of Pyrococcus furiosus RNase P, a multi-subunit catalytic ribonucleoprotein complex, by surface-induced dissociation and ion mobility mass spectrometry. Angew Chem Int Ed Engl. 2014 Oct 20;53(43):11483-7. doi: 10.1002/anie.201405362. Epub 2014 Sep 4. PMID: 25195671; PMCID: PMC4552314.
Lai LB, Bernal-Bayard P, Mohannath G, Lai SM, Gopalan V, Vioque A. A functional RNase P protein subunit of bacterial origin in some eukaryotes. Mol Genet Genomics. 2011 Dec;286(5-6):359-69. doi: 10.1007/s00438-011-0651-y. Epub 2011 Oct 11. PMID: 21987179.